Dietary cancer prevention with butyrate fermented by Aloe vera gel endophytic microbiota

ABSTRACT
AIM: Diet is a natural source of butyrate through the fermentation of non-digestive fiber, such as acemannan in Aloe vera gel, is a strong appearing target for health and quality of life as an immune modulation and colorectal cancer prevention in aged people. In our earlier research on fermentation by endophytic bacteria in Aloe vera gel, butyric acid was identified by GC/MSD analysis. Present investigation aims the identification of the microbiota. MATERIALS AND METHODS: The endophytic microbiota of Aloe vera gel in the fermented media were examined by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.
RESULTS: The following microbiota were identified: Bacillus cereus, B. licheniformis, Lactobacillus paralimentarium, Yeast: Clavispora lusitaniae. The safety pattern of the prepared Aloe vera gel was tested on normal non-cancerous cells and indicated the absence of any significant possible toxicity on the cells. Also, the extracted gels showed abilities to regulate the inflammatory responses in the inflammation cell models via the reduction in the amount of induced reactive oxygen species and both COX 1 and 2 enzymes.
DISCUSSION: Identification of butyrate-producing endophytic microbiota in Aloe vera gel fermentation and finding of inflammatory as well as antioxidant activities of butyrate in the fermented gel may help explain the known beneficial effects of butyrate in intestinal colon and on colitis. An innovative concept of symbiotics: a combination of Aloe vera gel juice and microbiota: Bacillus cereus, B.licheniformis. Lactobacillus paralimentarium and
Clavispora lusitaniae, is a perspective on alleviation of cancer disease and improvement of gastrointestinal health by butyrate fermentation.

要旨
「アロエベラ・ゲル内生菌の発酵酪酸によるがん予防食」

非栄養性食品の発酵を介した酪酸の産生、例えば、プレバイオティクスのアセマンナンの日常的摂取による腸内細菌による酪酸の産生は、腸管免疫能の亢進や大腸がんの予防に有効である。先に発表した論文の微生物はMALDI-TOF MS 分析により、以下と証明された。Bacillus cereus, B. Licheniformis, Lactobacillus paralimentarium, Clavispora lusitaniae.これら微生物の発酵液は細胞毒性なく抗酸化(ROS) とCoX 1 & 2 に対し免疫能活性を示した。

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